Developing a method to detect lipoarabinomannan in pleural fluid and assessing its diagnostic efficacy for tuberculous pleural effusion

ObjectivesThe diagnosis of tuberculosis pleural effusion (TPE) remains challenging, traditional diagnostic tests have limited efficacy. This study aimed to assess the performance fluid (PF) lipoarabinomannan (LAM) in TPE.MethodsA method for PF LAM (LAM-PF) was established using LEDBIO's AIMLAM kit. The LAM-PF evaluated 162 HIV-negative patients with suspected TPE.ResultsThe this exhibited good linearity and recovery rate, a limit detection (LOD) 2.90 pg/mL. Using cut-off value 5.33 pg/mL, sensitivity specificity diagnosing TPE (n = 128) were 47.7% 100.0%, respectively. probable 29) definite 99) 41.4% 49.5%, displayed significantly higher compared other methods. Combined testing adenosine deaminase (ADA)and increased 68.0%, area under curve 0.84 (0.77–0.89).ConclusionThis successfully detecting PF, which favorable TPE, particularly challenging cases TPE. ADA improves efficiency.